IVF Lab Service

Clinical embryology has put into practice in the laboratories for assisted reproduction techniques (ART) the recent discoveries regarding the physiology of early events in the development of human life. The embryologist is responsible for the correct and justified application of ART in these laboratories. Some of the guidelines which constitute the minimal requirements for any laboratory offering ART, with the aim of implementing a quality control system for all the embryologists of ESHRE. The strict application and further development of these guidelines will benefit all patients attending ART clinics.

The company offers pre-designed and engineered components with guaranteed performance and shorter assembly time. Below is the list of components offered by the company for IVF lab.

1. Laboratory design
The embryology laboratory should have adequate space to follow good laboratory practice.

     The construction of the laboratory should ensure aseptic and optimal handling of gametes and
       pre-embryos during all phases of the treatment.
     The location of storage areas and equipment such as incubators, centrifuges and cryo equipment should        be logically planned for efficiency and safety within each working area.
     Separate office space should be provided for administrative work, such as record keeping and data entry.
     A general wet area in which washing of equipment, sterilization, etc., is performed, should be separate        from the embryo laboratory. Moreover, if fixatives are applied, these analyses should be performed in a        separate room in a fume-hood.
     When commissioning the laboratory, thought should be given to the most recent developments in       equipment and facilities. Bench height, adjustable chairs, microscope eye height, efficient use of space       and surfaces, sufficient air-condition and the amount of daylight, all contribute to a working environment       that minimizes distraction and fatigue. Consideration should also be given to local health and safety       requirements.


2. Laboratory equipment

     The laboratory equipment used should be adequate for laboratory work and easy to clean and disinfect.
     Critical items of equipment, including incubators and frozen embryo storage facilities, should be       appropriately alarmed and monitored.
     All embryo laboratories should have an automatic emergency generator backup in the event of power        failure.
     A minimum number of two incubators is recommended. Gas cylinders should be placed outside or in a       separate room with an automatic backup system.
     Incubators should be frequently cleaned and sterilized. Nitrogen tanks should be cleaned and sanitized at       least every year.


3. Infectious agents

     All assisted reproductive technologies involve handling biological material, and pose a potential hazard of       transmitting diseases to personnel. Sources of infection in this system are compounds and follicle fluid of       animal origin. Therefore,

          ¤ Vaccination of the personnel against hepatitis B or other available viral disease is recommended.
          ¤ Screening patients and gamete donors for HIV, hepatitis B/C and other sexually transmitted diseases              before treatment or gamete cryopreservation should be routinely adopted.
          ¤ Although patients' admittance to IVF treatment cycles is regulated by physicians, the laboratory staff              must be informed about the risks of handling potentially infected biological material. The following              recommendations should be considered.

                 » If one or both partners are HIV-positive, IVF and embryo transfer should not be attempted, unless                     local rules give authorization after extensive examination. Donor semen is advised for a                     seronegative woman if her partner is seropositive, as HIV-1 viral particles may be present in the                     cytoplasmic compartment of motile spermatozoa. Therefore, the possibility exists that viral                     particles enter the oocyte during fertilization either with conventional IVF or intracytoplasmic                      sperm injection (ICSI). The fate of these particles in the resulting embryo is unknown.
                  » If the male partner is infected by the virus of hepatitis B, the seronegative woman must be                       vaccinated before IVF.
                  » If the woman is infected by the virus of hepatitis B, IVF is feasible but the couple must be warned                      about the necessity of specific vaccination of the baby at birth. It has been recently suggested                      that the use of ICSI in hepatitis B-positive patients could increase the risk of bringing the virus                      into the oocyte from the surrounding fluid, possibly adhered to the sperm membrane.
                  » If one partner is infected by the virus of hepatitis C, IVF may be performed after extensive                       counselling.
                  » In case of seropositivity for syphilis, appropriate treatment must be given before IVF.


4. Protective measures
The purpose of the protective measures is also to ensure aseptic conditions for gamete and embryos.
The procedures should deal with, but not be limited to, the following:

     Use of laboratory clothing.
     Use of non-toxic (non-powdered) gloves and masks.
     Use of eye and face protection if cryogenic materials are handled.
     Use of vertical laminar-flow benches.
     Use of mechanical pipetting devices.
     Use of mechanical pipetting devices.
     Disinfection and sterilization of potentially infected equipment.
     Use of disposable material; after usage, it must be discarded immediately in the proper waste containers.       Potential infectious materials must be disposed of in a manner that protects laboratory workers and       maintenance, service, and housekeeping staff from exposure to infectious materials in the course of their       work.
     Needles and other sharps should be handled with extreme caution and discarded in special containers.

Cross-contamination with infectious material from one patient to another could still occur during the cryopreservation procedure when straws with semen or embryos are filled by dipping the straw in patient medium with semen or embryos, sealing it and passing it into liquid nitrogen without external disinfection. Material stored in the cryopreservation tanks should be kept in a way that avoids contact of the liquid nitrogen phase with the biological substances. Safety cryo straws have been conceived in order to fulfil this requirement. Specimen known to be contaminated should be stored in such high security straws.













 

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